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WB analysis of MCF-7 using 24102-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 24102-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 24102-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 24102-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 24102-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 24102-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 24102-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 24102-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NT5DC1 antibody (24102-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

NT5DC1 Polyclonal antibody

Name: NT5DC1 Polyclonal antibody
Brand: Proteintech
SKU: 24102-1-AP

NT5DC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24102-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

NT5C2L1, EC:3.1.3.-, 5'-nucleotidase domain-containing protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	MCF7 cells, HepG2 cells, Jurkat cells
Positive IHC detected in	human lung cancer tissue, human thyroid cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IP	See 1 publications below
ChIP	See 1 publications below

Product Information

24102-1-AP targets NT5DC1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20916 Product name: Recombinant human NT5DC1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-297 aa of BC015138 Sequence: WKDIVAAIQHNYKMSAFKENCGIYFPEIKRDPGRYLHSCPESVKKWLRQLKNAGKILLLITSSHSDYCRLLCEYILGNDFTDLFDIVITNALKPGFFSHLPSQRPFRTLENDEEQEALPSLDKPGWYSQGNAVHLYELLKKMTGKPEPKVVYFGDSMHSDIFPARHYSNWETVLILEELRGDEGTRSQRPEESEPLEKKGKYEGPKAKPLNTSSKKWGSFFIDSVLGLENTEDSLVYTWSCKRISTYSTIAIPSIEAIAELPLDYKFTRFSSSNSKTAGYYPNPPLVLSSDETLISK Predict reactive species
Full Name	5'-nucleotidase domain containing 1
Calculated Molecular Weight	455 aa, 52 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC015138
Gene Symbol	NT5DC1
Gene ID (NCBI)	221294
RRID	AB_2879431
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q5TFE4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for NT5DC1 antibody 24102-1-AP	Download protocol
IHC protocol for NT5DC1 antibody 24102-1-AP	Download protocol
WB protocol for NT5DC1 antibody 24102-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB,IP,ChIP	Nat Commun PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression. Authors - Xianchun Lan View Article