Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, HeLa cells, K-562 cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IP | See 1 publications below |
Product Information
11154-1-AP targets NTHL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1631 Product name: Recombinant human NTHL1 protein Source: e coli.-derived, Unknow Tag: N/A Domain: C16022 of BC003014 Sequence: Predict reactive species |
| Full Name | nth endonuclease III-like 1 (E. coli) |
| Calculated Molecular Weight | 34 aa, 3 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC003014 |
| Gene Symbol | NTHL1 |
| Gene ID (NCBI) | 4913 |
| RRID | AB_2283005 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78549 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NTHL1 antibody 11154-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB J Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats. | ||
Respir Res Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase. | ||
NAR Cancer The DNA repair function of BCL11A suppresses senescence and promotes continued proliferation of triple-negative breast cancer cells | ||
Nucleic Acids Res The function of BCL11B in base excision repair contributes to its dual role as an oncogene and a haplo-insufficient tumor suppressor gene |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH James (Verified Customer) (02-17-2023) | Recognises NTHL1 at 34kDa, but has non-specific bands which decrease with siRNA knock-down of NTHL1 at higher molecular weights (150kDa).
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