Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14373-1-AP targets NTM in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5746 Product name: Recombinant human NTM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-316 aa of BC050716 Sequence: VCGYLFLPWKCLVVVSLRLLFLVPTGVPVRSGDATFPKAMDNVTVRQGESATLRCTIDNRVTRVAWLNRSTILYAGNDKWCLDPRVVLLSNTQTQYSIEIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVSPKIVEISSDISINEGNNISLTCIATGRPEPTVTWRHISPKAVGFVSEDEYLEIQGITREQSGDYECSASNDVAAPVVRRVKVTVNYPPYISEAKGTGVPVGQKGTLQCEASAVPSAEFQWYKDDKRLIEGKKGVKVENRPFLSKLIFFNVSEHDYGNYTCVASNKLGHTNASIMLFGETVL Predict reactive species |
| Full Name | neurotrimin |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 58 kDa, 65 kDa |
| GenBank Accession Number | BC050716 |
| Gene Symbol | NTM |
| Gene ID (NCBI) | 50863 |
| RRID | AB_2878048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P121 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neurotrimin (NTM) is a glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecule. Neurotrimin together with the limbic system-associated protein (LAMP) and the opioid binding cell adhesion molecule (OBCAM) comprise the IgLON family of the immunoglobulin superfamily. Neurotrimin may be promoting neurite outgrowth and adhesion via a homophilic mechanism.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NTM antibody 14373-1-AP | Download protocol |
| IHC protocol for NTM antibody 14373-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
