NUAK2 Antibody
Rabbit Polyclonal
Catalog number: 11592-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | Y79 cells, HeLa cells |
| Positive IHC detected in | human kidney |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
NIH3T3 cells on cover slips fixed and staining with anti-NUAK2 (11592-1-AP,1:100;Rabbit polyclonal -Red) and anti-pericentrin(centrosome Marker; McAb-Green). The photo shows the centrosome in cell cycle: Interphase, Prophase, Prometaphase, Metaphase, Anaphase and Telophase. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml.(from Dr. Zineldeen Doaa Hussein) 
Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP(NUAK2 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human kidney using 11592-1-AP(NUAK2 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney using 11592-1-AP(NUAK2 Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | NUAK2 fusion protein ag2031 | Full name | NUAK family, SNF1-like kinase, 2 |
|
Calculated molecular weight |
628aa,70kd |
Observed molecular
weight |
70kd |
|
GenBank accession number |
BC017306 | Gene ID (NCBI) | 81788 |
| Gene symbol | NUAK2 | Synonyms | DKFZp434J037; DKFZp686F01113; FLJ90349; SNARK |
Background
NUAK2(NUAK family SNF1-like kinase 2) is also named as OMPHK2, SNARK and belongs to the SNF1 subfamily. The function of NUAK2 is partly dependent on anti-apoptotic properties against apoptosis induced by death ligand and NUAK2 also has effects on the migration of cancer cells as speculated from studies on myosin filaments and cytoskeleton organization in normal cells(PMID:21911917). Western blot analysis detected NUAK2 expressed in cancer cell lines of diverse tissue origin(PMID:12409306). This antibody is specific to NUAK2. |
