• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

NUB1 Polyclonal antibody

NUB1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14343-1-AP

Synonyms

BS4, NEDD8 ultimate buster 1, NUB1, NUB1L, NYREN18

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using NUB1 Polyclonal antibody (14343-1-AP)

    WB analysis of HeLa using 14343-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NUB1 Polyclonal antibody (14343-1-AP)

    WB analysis of HEK-293 using 14343-1-AP

    WB result of NUB1 antibody (14343-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUB1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of Raji cells using NUB1 Polyclonal antibody (14343-1-AP)

    WB analysis of Raji using 14343-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using NUB1 Polyclonal antibody (14343-1-AP)

    WB analysis of mouse kidney using 14343-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using NUB1 Polyclonal antibody (14343-1-AP)

    WB analysis of COLO 320 using 14343-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NUB1 Polyclonal antibody (14343-1-AP)

    WB analysis of HepG2 using 14343-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NUB1 Polyclonal antibody (14343-1-AP)

    WB analysis of K-562 using 14343-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NUB1 Polyclonal antibody (14343-1-AP)

    IP experiment of HEK-293 using 14343-1-AP

    IP result of anti-NUB1 (IP:14343-1-AP, 3ug; Detection:14343-1-AP 1:600) with HEK-293 cells lysate 2500ug.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NUB1 Polyclonal antibody (14343-1-AP)

    IP experiment of HEK-293 using 14343-1-AP

    IP result of anti-NUB1 (IP:14343-1-AP, 4ug; Detection:14343-1-AP 1:600) with HEK-293 cells lysate 1575 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUB1 Polyclonal antibody (14343-1-AP)

    IF Staining of HeLa using 14343-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUB1 antibody (14343-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, Raji cells, mouse kidney tissue, COLO 320 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14343-1-AP targets NUB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUB1 fusion protein Ag5668
Full Name negative regulator of ubiquitin-like proteins 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC046354
Gene Symbol NUB1
Gene ID (NCBI) 51667
RRIDAB_2155889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteasome-associated protein NEDD8 ultimate buster 1 (NUB1) is an interferon-inducible protein that down-regulates NEDD8 expression and its conjugation system. Over-expression of NUB1 induces a growth-inhibitory effect in cells, and it mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Expression of NUB1 leads to decreased modification of p53 with NEDD8 and stimulation of p53 ubiquitination. Recent finding revealed that NUB1, by regulating GSK3β levels, modulates tau phosphorylation and aggregation, and is a key player in neurodegeneration associated with tau pathology.

Protocols

Product Specific Protocols
WB protocol for NUB1 antibody 14343-1-APDownload protocol
IF protocol for NUB1 antibody 14343-1-APDownload protocol
IP protocol for NUB1 antibody 14343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Biochem Biophys Res Commun

The role of NUB1 in α-synuclein degradation in Lewy body disease model mice.

Authors - Kunikazu Tanji
View Article
humanIHC

Neuropathology

Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.

Authors - Fumiaki Mori
View Article
humanWB

Brain Pathol

Phosphorylated NUB1 distinguishes α-synuclein in Lewy bodies from that in glial cytoplasmic inclusions in multiple system atrophy

Authors - Kunikazu Tanji
View Article