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WB analysis using 16098-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16098-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16098-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human colon cancer using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 16098-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

NUDT8 Polyclonal antibody

Name: NUDT8 Polyclonal antibody
Brand: Proteintech
SKU: 16098-1-AP

NUDT8 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16098-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:3.6.1.-, Mitochondrial coenzyme A diphosphatase NUDT8, Nucleoside diphosphate-linked moiety X motif 8, Nudix motif 8

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells
Positive IHC detected in	human colon cancer tissue, human heart tissue, human kidney tissue, human skin tissue, mouse heart tissue, human liver tissue, human spleen tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16098-1-AP targets NUDT8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NUDT8 fusion protein Ag8945 Predict reactive species
Full Name	nudix (nucleoside diphosphate linked moiety X)-type motif 8
Calculated Molecular Weight	236 aa, 25 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC018644
Gene Symbol	NUDT8
Gene ID (NCBI)	254552
RRID	AB_2154132
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WV74
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT8 belongs to the Nudix hydrolase family, which is involved in intracellular metabolic processes, especially related to the decomposition and degradation of nucleotides. NUDT8 is a novel CoA diphosphohydrolase that localizes to the mitochondria and is broadly expressed in mitochondria-rich organs such as the heart, kidneys, liver, and brown adipose tissue(PMID: 31004344).