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NUP160 Polyclonal antibody

NUP160 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 16084-1-AP

Synonyms

160 kDa nucleoporin, KIAA0197, nucleoporin 160kDa, Nucleoporin Nup160, NUP120, NUP160

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NUP160 Polyclonal antibody (16084-1-AP)

    WB analysis of HeLa using 16084-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16084-1-AP (NUP160 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NUP160 Polyclonal antibody (16084-1-AP)

    WB analysis of human brain using 16084-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16084-1-AP (NUP160 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NUP160 Polyclonal antibody (16084-1-AP)

    IP experiment of HeLa using 16084-1-AP

    IP result of anti-NUP160 (IP:16084-1-AP, 4ug; Detection:16084-1-AP 1:500) with HeLa cells lysate 4000ug.

Tested Applications

Positive WB detected inHeLa cells, human brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16084-1-AP targets NUP160 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP160 fusion protein Ag9083
Full Name nucleoporin 160kDa
Calculated Molecular Weight 1316 aa, 149 kDa
Observed Molecular Weight 149 kDa
GenBank Accession NumberBC009822
Gene Symbol NUP160
Gene ID (NCBI) 23279
RRIDAB_2157341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NUP160 antibody 16084-1-APDownload protocol
IP protocol for NUP160 antibody 16084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols