NUP205 Polyclonal antibody

NUP205 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24439-1-AP

Synonyms

205 kDa nucleoporin, C7orf14, KIAA0225, nucleoporin 205kDa, Nucleoporin Nup205, NUP205



Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24439-1-AP is NUP205 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP205 fusion protein Ag19832
Full Name nucleoporin 205kDa
Calculated Molecular Weight 2012 aa, 228 kDa
Observed Molecular Weight 205 kDa
GenBank Accession NumberBC136624
Gene Symbol NUP205
Gene ID (NCBI) 23165
RRIDAB_2879550
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUP205, also named as C7orf14 and KIAA0225, is a 205 kDa nucleoporin. It plays a role in the nuclear pore complex (NPC) assembly and/or maintenance.

Protocols

Product Specific Protocols
WB protocol for NUP205 antibody 24439-1-APDownload protocol
IHC protocol for NUP205 antibody 24439-1-APDownload protocol
IP protocol for NUP205 antibody 24439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte
mouseWB

J Clin Invest

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Authors - Zhixiong Liu
humanWB

Oncogene

DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Authors - Bin Chen
humanWB

Hum Mutat

Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation.

Authors - Meng Han
mouseIF

Cell Rep Methods

TissUExM enables quantitative ultrastructural analysis in whole vertebrate embryos by expansion microscopy

Authors - Emmanuelle Steib
humanWB,IHC

Front Oncol

Exploring the relationship between abnormally high expression of NUP205 and the clinicopathological characteristics, immune microenvironment, and prognostic value of lower-grade glioma

Authors - Wenjia Liang