gp210 Polyclonal antibody

gp210 Polyclonal Antibody for WB, IP, ELISA

Cat No. 16909-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

NUP210, KIAA0906, Nuclear pore protein gp210, nucleoporin 210kDa, Nucleoporin Nup210

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIP

WB analysis of mouse lung using 16909-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16909-1-AP (gp210 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 16909-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16909-1-AP (gp210 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse thymus using 16909-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16909-1-AP (gp210 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 16909-1-AP
IP result of anti-gp210 (IP:16909-1-AP, 4ug; Detection:16909-1-AP 1:300) with HeLa cells lysate 3040 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	mouse lung tissue, mouse thymus tissue, unboiled HEK-293 cells
Positive IP detected in	HeLa cells

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

16909-1-AP targets gp210 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10533 Product name: Recombinant human NUP210 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 10-351 aa of BC067089 Sequence: LLTLSVLLAAGPSAAAAKLNIPKVLLPFTRATRVNFTLEASEGCYRWLSTRPEVASIEPLGLDEQQCSQKAVVQARLTQPARLTSIIFAEDITTGQVLRCDAIVDLIHDIQIVSTTRELYLEDSPLELKIQALDSEGNTFSTLAGLVFEWTIVKDSEADRFSDSHNALRILTFLESTYIPPSYISEMEKAAKQGDTILVSGMKTGSSKLKARIQEAVYKNVRPAEVRLLILENILLNPAYDVYLMVGTSIHYKVQKIRQGKITELSMPSDQYELQLQNSIPGPEGDPARPVAVLAQDTSMVTALQLGQSSLVLGHRSIRMQGASRLPNSTIYVVEPGYLGFT Predict reactive species
Full Name	nucleoporin 210kDa
Calculated Molecular Weight	967aa,106 kDa; 1887aa,205 kDa
Observed Molecular Weight	205 kDa
GenBank Accession Number	BC067089
Gene Symbol	gp210
Gene ID (NCBI)	23225
RRID	AB_2157365
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TEM1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IP protocol for gp210 antibody 16909-1-AP	Download protocol
WB protocol for gp210 antibody 16909-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Mutat Res METTL3 promotes prostate cancer cell metastasis and EMT by mediating NUP210 m6A modification. Authors - Shaoxing Wang KD Validated View Article