NUP53 Polyclonal antibody

NUP53 Polyclonal Antibody for WB, ELISA

Cat No. 19819-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

35 kDa nucleoporin, Mitotic phosphoprotein 44, MP 44, MP44, nucleoporin 35kDa, Nucleoporin Nup35, Nucleoporin NUP53, NUP35, NUP53

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis using 19819-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19819-1-AP (NUP53 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of K-562 using 19819-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 19819-1-AP (NUP53 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

HEK-293 cells, K-562 cells, HeLa cells, HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

19819-1-AP targets NUP53 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13869 Product name: Recombinant human NUP35 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-326 aa of BC061698 Sequence: MAAFAVEPQGPALGSEPMMLGSPTSPKPGVNAQFLPGFLMGDLPAPVTPQPRSISGPSVGVMEMRSPLLAGGSPPQPVVPAHKDKSGAPPVRSIYDDISSPGLGSTPLTSRRQPNISVMQSPLVGVTSTPGTGQSMFSPASIGQPRKTTLSPAQLDPFYTQGDSLTSEDHLDDSWVTVFGFPQASASYILLQFAQYGNILKHVMSNTGNWMHIRYQSKLQARKALSKDGRIFGESIMIGVKPCIDKSVMESSDRCALSSPSLAFTPPIKTLGTPTQPGSTPRISTMRPLATAYKASTSDYQVISDRQTPKKDESLVSKAMEYMFGW Predict reactive species
Full Name	nucleoporin 35kDa
Calculated Molecular Weight	35 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	BC061698
Gene Symbol	NUP53
Gene ID (NCBI)	129401
RRID	AB_2878611
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NFH5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for NUP53 antibody 19819-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human		bioRxiv Enterovirus D68 2A protease causes nuclear pore complex dysfunction and motor neuron toxicity Authors - Katrina M Zinn View Article
human	WB	Elife Enterovirus D68 2A protease causes nuclear pore complex dysfunction and independently contributes to motor neuron toxicity. Authors - Katrina M Zinn View Article
human	WB	Life Sci TRAF2 mediates Wnt-induced β-catenin nuclear translocation by associating with the nuclear pore complex Authors - Min Yang View Article