NXF1 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB |
| Cited applications | CoIP, FC, IF, IP, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, monkey |
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Recommended dilution | WB: 1:200-1:2000 |
IP = Immunoprecipitation
HeLa cells were subjected to SDS PAGE followed by western blot with 10328-1-AP(NXF1 Antibody) at dilution of 1:600
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | NXF1 fusion protein ag0413 | Full name | nuclear RNA export factor 1 |
|
Calculated molecular weight |
70kd |
Observed molecular
weight |
66-70kd |
|
GenBank accession number |
BC004904 | Gene ID (NCBI) | 10482 |
| Gene symbol | NXF1 | Synonyms | DKFZp667O0311; MEX67; TAP |
Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. |
