Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13275-1-AP targets NXF3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3998 Product name: Recombinant human NXF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC031616 Sequence: MSLPSGHTTGHTDQVVQRRARCWDIYQRRFSSRSEPVNPGMHSSSHQQQDGDAAMHGAHMDSPVRYTPYTISPYNRKGSFRKQDQTHVNMEREQKPPERRMEGNMPDGTLGSWFKITVPFGIKYNEKWLLNLIQNECSVPFVPVEFHYENMHASFFVENASIAYALKNVSGKIWDEDNEKISIFVNPAGIPHFVHRELKSEKVEQIKLAMNQQCDVSQEALDIQRLPFYPDMVNRDTKMASNPRKCMAASLDVHEENIPTVMSAGEMDKWKGIEPGEKCADRSPVCTTFSDTSSNINSILELF Predict reactive species |
| Full Name | nuclear RNA export factor 3 |
| Calculated Molecular Weight | 531 aa, 60 kDa |
| GenBank Accession Number | BC031616 |
| Gene Symbol | NXF3 |
| Gene ID (NCBI) | 56000 |
| RRID | AB_2877933 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H4D5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NXF3 (nuclear RNA export factor 3) belongs to the NXF family of RNA transport proteins, predominantly expressed in human testis cells. Unlike canonical NXF1, it bears truncated functional domains and lacks typical RNA-binding capacity. It mediates nucleocytoplasmic shuttling of snoRNAs and regulates TGF-β3 mRNA trafficking independent of bulk mRNA export. Recent studies confirm its oncogenic role in gastric cancer, where elevated NXF3 accelerates tumor proliferation, invasion and predicts poor patient survival. It acts as a prognostic biomarker and a potential therapeutic target for gastrointestinal malignancies. (PMID: 29021253)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NXF3 antibody 13275-1-AP | Download protocol |
| WB protocol for NXF3 antibody 13275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci Nuclear translocation of CDK5RAP3 regulated by NXF3 promotes the progression of gastric cancer | ||
Sci Transl Med A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins |
