NeuN Polyclonal antibody

NeuN Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23060-1-AP

Synonyms



Tested Applications

Positive WB detected inmouse brain tissue, 3T3-L1 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23060-1-AP is NeuN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, goat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NeuN fusion protein Ag19347
Full Name hexaribonucleotide binding protein 3
Observed Molecular Weight 38-50 kDa
GenBank Accession NumberBC093713
Gene Symbol hCG_1776007
Gene ID (NCBI) 146713
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events.

Publications

SpeciesApplicationTitle
goatIF

Cell Cycle

Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.

Authors - Zhimin Wang
hamsterIHC

Neurotherapeutics

PMID: 28083805

Authors - Syed Zahid Ali Shah
ratWB,IF

Neurochem Int

Tert-butylhydroquinone post-treatment attenuates neonatal hypoxic-ischemic brain damage in rats.

Authors - Juan Zhang
mouseIF

J Stroke Cerebrovasc Dis

An Increase of Sigma-1 Receptor in the Penumbra Neuron after Acute Ischemic Stroke.

Authors - Xiaotian Zhang
humanIHC

Pediatr Surg Int

Pathological changes of interstitial cells of Cajal and ganglion cells in the segment of resected bowel in Hirschsprung's disease.

Authors - Xuyong Chen
mouse

Neurochem Int

Photobiomodulation treatment inhibits neurotoxic astrocytic polarization and protects neurons in in vitro and in vivo stroke models

Authors - Yu Feng