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NUDCD3 Polyclonal antibody

NUDCD3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11764-1-AP

Synonyms

KIAA1068, NudC domain containing 3, NUDCD3, NudCL

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using NUDCD3 Polyclonal antibody (11764-1-AP)

    WB analysis of human liver using 11764-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11764-1-AP (NUDCD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NUDCD3 Polyclonal antibody (11764-1-AP)

    WB analysis of HEK-293 using 11764-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11764-1-AP (NUDCD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NUDCD3 Polyclonal antibody (11764-1-AP)

    WB analysis of human brain using 11764-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11764-1-AP (NUDCD3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NUDCD3 Polyclonal antibody (11764-1-AP)

    WB analysis of HeLa using 11764-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11764-1-AP (NUDCD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NUDCD3 Polyclonal antibody (11764-1-AP)

    IP experiment of mouse brain using 11764-1-AP

    IP result of anti-NUDCD3 (IP:11764-1-AP, 4ug; Detection:11764-1-AP 1:800) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using NUDCD3 Polyclonal antibody (11764-1-AP)

    IHC staining of human brain using 11764-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 11764-1-AP (NUDCD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NUDCD3 Polyclonal antibody (11764-1-AP)

    IHC staining of human brain using 11764-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 11764-1-AP (NUDCD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUDCD3 Polyclonal antibody (11764-1-AP)

    IF Staining of HeLa using 11764-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11764-1-AP (NUDCD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman liver tissue, HEK-293 cells, HeLa cells, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11764-1-AP targets NUDCD3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDCD3 fusion protein Ag2362
Full Name NudC domain containing 3
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC003691
Gene Symbol NUDCD3
Gene ID (NCBI) 23386
RRIDAB_2298665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NUDCD3 antibody 11764-1-APDownload protocol
IHC protocol for NUDCD3 antibody 11764-1-APDownload protocol
IF protocol for NUDCD3 antibody 11764-1-APDownload protocol
IP protocol for NUDCD3 antibody 11764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration.

Authors - Min Liu
View Article