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WB analysis using 19842-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19842-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 19842-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19842-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19842-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19842-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 19842-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 19842-1-AP
IP result of anti-hIST1 (IP:19842-1-AP, 4ug; Detection:19842-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IHC staining of mouse lung using 19842-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 19842-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 19842-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 19842-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using hIST1 antibody (19842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

hIST1 Polyclonal antibody

Name: hIST1 Polyclonal antibody
Brand: Proteintech
SKU: 19842-1-AP
Rating: 5.00 (1 reviews)

hIST1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19842-1-AP

Publications(11)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Synonyms

IST1, KIAA0174, Charged multivesicular body protein 8, CHMP8, IST1 homolog

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (11)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, mouse liver tissue, mouse brain tissue, PC-3 cells
Positive IHC detected in	mouse stomach tissue, mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 8 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

19842-1-AP targets hIST1 in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13874 Product name: Recombinant human hIST1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC000116 Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFEELKKKT Predict reactive species
Full Name	KIAA0174
Calculated Molecular Weight	364 aa, 40 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	BC000116
Gene Symbol	hIST1
Gene ID (NCBI)	9798
RRID	AB_2878612
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P53990
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for hIST1 antibody 19842-1-AP	Download protocol
IHC protocol for hIST1 antibody 19842-1-AP	Download protocol
IF protocol for hIST1 antibody 19842-1-AP	Download protocol
IP protocol for hIST1 antibody 19842-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nature A phosphoinositide signalling pathway mediates rapid lysosomal repair Authors - Jay Xiaojun Tan View Article
	IF	Science Triggered recruitment of ESCRT machinery promotes endolysosomal repair. Authors - Michael L Skowyra View Article
human	IF	Proc Natl Acad Sci U S A Ca2+-sensor ALG-2 engages ESCRTs to enhance lysosomal membrane resilience to osmotic stress Authors - Wei Chen View Article
human	WB	Dev Cell NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C. Authors - Oliver B Davis View Article
human		MBio Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response. Authors - Ekansh Mittal View Article
mouse	WB,RIP,IF	J Neurochem Reduced circ_lrrc49 in trigeminal ganglion contributes to neuropathic pain in mice by downregulating Ist1 and impairing autophagy Authors - Qingfeng Tang View Article