Tested Applications
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10079-1-AP targets OLFM1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0125 Product name: Recombinant human AMY protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-169 aa of BC000189 Sequence: MPGRWRWQRDMHPARKLLSLLFLILMGTELTQNKRENKAEKMGGPESERKTTGEKTLNELPLFCLEAHAGSLALPRMCSPNPNPAVGLCRPAYPQSPSPGAAQTISQSLLERFCMASRREVFLAPGRPGGGWWLCTVAGQMHSFMCTHTHTHAHTGEQIPAEKSQPGPD Predict reactive species |
| Full Name | olfactomedin 1 |
| Calculated Molecular Weight | 20 kDa |
| GenBank Accession Number | BC000189 |
| Gene Symbol | OLFM1 |
| Gene ID (NCBI) | 10439 |
| RRID | AB_2157220 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99784 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Olfactomedin 1 (Olfm1), also known as noelin and pancortin, is a secreted glycoprotein belonging to a family of olfactomedin domain-containing proteins. The gene of Olfm1 is located on chromosome 9q34.3. Four variant transcripts (AMY, BMY, AMZ, and BMZ) are produced by alternative splicing. Olfm1 may be involved in the regulation of neural crest cell production, neuronal differentiation and ischemic neuronal death in adult. This antibody is specific for isoform 3 and isoform 4 (AMY) of Olfm1 with native molecular weights of 54 kDa and 16 kDa respectively. AMY may have an essential role in nerve tissue.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for OLFM1 antibody 10079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
