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  • Western Blot (WB) analysis of HepG2 cells using ORC2L Polyclonal antibody (12739-1-AP)

    WB analysis of HepG2 using 12739-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12739-1-AP (ORC2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ORC2L Polyclonal antibody (12739-1-AP)

    WB analysis of HepG2 using 12739-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12739-1-AP (ORC2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ORC2L Polyclonal antibody (12739-1-AP)

    WB analysis of HepG2 using 12739-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12739-1-AP (ORC2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ORC2L Polyclonal antibody (12739-1-AP)

    IP experiment of HepG2 using 12739-1-AP

    IP result of anti-ORC2L (IP:12739-1-AP, 4ug; Detection:12739-1-AP 1:300) with HepG2 cells lysate 1200ug.

ORC2L Polyclonal antibody

ORC2L Polyclonal Antibody for WB, IP, ELISA

Cat No. 12739-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

ORC2, ORC2L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12739-1-AP targets ORC2L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3635

Product name: Recombinant human ORC2L protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 208-578 aa of BC014834

Sequence: SQKIQAQNRVVSAPVGKETPSKRMKRDKTSDLVEEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHKWMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVLDHMGTFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYLIASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHVLRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIPVDNGTLTDFLEKEEEEA

Predict reactive species
Full Name origin recognition complex, subunit 2-like (yeast)
Calculated Molecular Weight 577 aa, 66 kDa
Observed Molecular Weight 70-74 kDa
GenBank Accession NumberBC014834
Gene Symbol ORC2L
Gene ID (NCBI) 4999
RRIDAB_10640799
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13416
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ORC2L antibody 12739-1-APDownload protocol
IP protocol for ORC2L antibody 12739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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