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  • Western Blot (WB) analysis of HeLa cells using ORC4L Polyclonal antibody (13026-1-AP)

    WB analysis of HeLa using 13026-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13026-1-AP (ORC4L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ORC4L Polyclonal antibody (13026-1-AP)

    IF Staining of HepG2 using 13026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 13026-1-AP (ORC4L antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ORC4L Polyclonal antibody (13026-1-AP)

    IF Staining of HeLa using 13026-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13026-1-AP (ORC4L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ORC4L Polyclonal antibody

ORC4L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13026-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ORC4P, ORC4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13026-1-AP targets ORC4L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3625

Product name: Recombinant human ORC4L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 85-437 aa of BC014847

Sequence: MEIEEVSENVLQVHLNGLLQINDKIALKEITRQLNLENVVGDKVFGSFAENLSFLLEALKKGDRTSSCPVIFILDEFDLFAHHKNQTLLYNLFDISQSAQTPIAVIGLTCRLDILELLEKRVKSRFSHRQIHLMNSFGFPQYVKIFKEQLSLPAEFPDKVFAEKWNENVQYLSEDRSVQEVLQKHFNISKNLRSLHMLLMLALNRVTASHPFMTAVDLMEASQLCSMDSKANIVHGLSVLEICLIIAMKHLNDIYEEEPFNFQMVYNEFQKFVQRKAHSVYNFEKPVVMKAFEHLQQLELIKPMERTSGNSQREYQLMKLLLDNTQIMNALQKYPNCPTDVRQWATSSLSWL

Predict reactive species
Full Name origin recognition complex, subunit 4-like (yeast)
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC014847
Gene Symbol ORC4L
Gene ID (NCBI) 5000
RRIDAB_2157873
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43929
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ORC4L antibody 13026-1-APDownload protocol
WB protocol for ORC4L antibody 13026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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