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WB analysis using 15033-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 15033-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15033-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 15033-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 15033-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 15033-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15033-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 15033-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 15033-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 40x lens).

OSGEP Polyclonal antibody

Name: OSGEP Polyclonal antibody
Brand: Proteintech
SKU: 15033-1-AP

OSGEP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15033-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

GCPL1, hOSGEP, KAE1, OSGEP, OSGEP1, PRSMG1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	PC-3 cells, human heart tissue, Raji cells, mouse colon tissue
Positive IHC detected in	human kidney tissue, human heart tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

15033-1-AP targets OSGEP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4308 Product name: Recombinant human OSGEP protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-335 aa of BC032310 Sequence: MPAVLGFEGSANKIGVGVVRDGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTESGLTSQDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLVGVNHCIGHIEMGRLITGATSPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMDVSFSGILSFIEDVAHRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSQEALIVGGVGCNVRLQEMMATMCQERGARLFATDERFCIDNGAMIAQAGWEMFRAGHRTPLSDSGVTQRYRTDEVEVTWRD Predict reactive species
Full Name	O-sialoglycoprotein endopeptidase
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC032310
Gene Symbol	OSGEP
Gene ID (NCBI)	55644
RRID	AB_2283171
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NPF4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OSGEP(O-sialoglycoprotein endopeptidase) is also named as hOSGEP, GCPL1 and belongs to the KAE1 / YgjD family. The deduced 335-amino acid OSGEP protein has a predicted molecular mass of 36.4 kDa. It is a protease whose target is O-linked sialic acid-containing glycoproteins, such as episialin(PMID:10859271). It is involved in the metabolism of glycated proteins as a glycoprotein-related chaperon protease(PMID: 20824107).

Protocols

Product Specific Protocols
IHC protocol for OSGEP antibody 15033-1-AP	Download protocol
WB protocol for OSGEP antibody 15033-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nucleic Acids Res Commonality and diversity in tRNA substrate recognition in t6A biogenesis by eukaryotic KEOPSs. Authors - Jin-Tao Wang View Article