OTX2 Antibody
Rabbit Polyclonal
Catalog number: 13497-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, FC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | Y79 cells |
| Positive IHC detected in | human gliomas |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
FC result of anti-OTX2 (13497-1-AP, 1:100) with hESC which spontaneous differentiation toward retinal lineage (RPE). (Black: control; Blue OTX2).
Y79 cells were subjected to SDS PAGE followed by western blot with 13497-1-AP(OTX2 Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human gliomas using 13497-1-AP(OTX2 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human gliomas using 13497-1-AP(OTX2 Antibody) at Dilution 1:100 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | OTX2 fusion protein ag4323 | Full name | orthodenticle homeobox 2 |
|
Calculated molecular weight |
297aa,32kd |
Observed molecular
weight |
32kd |
|
GenBank accession number |
BC032579 | Gene ID (NCBI) | 5015 |
| Gene symbol | OTX2 | Synonyms | MCOPS5; MGC45000 |
Background
The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive |
