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WB analysis of human kidney using 14943-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 14943-1-AP (OVCA2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14943-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14943-1-AP (OVCA2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14943-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14943-1-AP (OVCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 14943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14943-1-AP (OVCA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 14943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14943-1-AP (OVCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OVCA2 Polyclonal antibody

OVCA2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14943-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:3.1.2.-, Esterase OVCA2, Ovarian cancer-associated gene 2 protein, OVCA 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	human kidney tissue, human brain tissue, mouse thymus tissue
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

KD/KO	See 1 publications below
WB	See 1 publications below

14943-1-AP targets OVCA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6744 Product name: Recombinant human OVCA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC041170 Sequence: MAAQRPLRVLCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVPDPPGPEGARSDFGSCPPEEQPRGWWFSEQEADVFSALEEPAVCRGLEESLGMVAQALNRLGPFDGLLGFSQGAALAALVCALGQAGDPRFPLPRFILLVSGFCPRGIGFKESILQRPLSLPSLHVFGDTDKVIPSQESVQLASQFPGAITLTHSGGHFIPAAAPQRQAYLKFLDQFAE Predict reactive species
Full Name	candidate tumor suppressor in ovarian cancer 2
Calculated Molecular Weight	24 kDa
Observed Molecular Weight	28 kDa
GenBank Accession Number	BC041170
Gene Symbol	OVCA2
Gene ID (NCBI)	124641
RRID	AB_2157286
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WZ82
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for OVCA2 antibody 14943-1-AP	Download protocol
WB protocol for OVCA2 antibody 14943-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Hematology OVCA2 acts as an oncogene in pediatric AML by negatively regulating CDKN1A to drive cell cycle progression. Authors - Wanyan Jiao KD Validated View Article