OXSR1 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells, HEK-293 cells, human liver tissue, human testis tissue |
| Positive IHC detected in | human prostate cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:50 |
IP = Immunoprecipitation
HeLa cells were subjected to SDS PAGE followed by western blot with 15611-1-AP(OXSR1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate cancer using 15611-1-AP(OXSR1 Antibody) at Dilution 1:100 (under 40x lens)
Immunohistochemistry of paraffin-embedded human prostate cancer using 15611-1-AP(OXSR1 Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using OXSR1 antibody 15611-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | OXSR1 fusion protein ag8002 | Full name | oxidative-stress responsive 1 |
|
Calculated molecular weight |
58kd |
Observed molecular
weight |
58kd |
|
GenBank accession number |
BC008726 | Gene ID (NCBI) | 9943 |
| Gene symbol | OXSR1 | Synonyms | KIAA1101; OSR1 |
Oxidative-stress responsive 1(OXSR1) is also named as KIAA1101, OSR1 and belongs to the STE Ser/Thr protein kinase family. It contains an N-terminal Ste20-like ser/thr kinase domain and 2 C-terminal regions, which has a putative caspase-3 cleavage site at the end. OXSR1's interaction with WNK1 is required for NKCC function, and it modulates the G protein sensitivity of PAK by phosphorylation of PAK1.Western blot analysis detected Oxsr1 at an apparent molecular mass of 58 kD in all mouse tissues examined except thymus. Cell fractionation and immunofluorescence analysis of HeLa cells showed that OXSR1 was distributed throughout the cell and OXSR1 could phosphorylate a test substrate and itself(PMID:14707132). This antibody is specific to OXSR1. |
