P53 Antibody
Rabbit Polyclonal
Catalog number: 10442-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | A431 cells, HEK-293 cells, MCF7 cells |
| Positive IHC detected in | human endometrial cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-TP53 (10442-1-AP) with A431 cell.
WB result of anti-TP53 (10442-1-AP) in different cell lysates.
A431 cells were subjected to SDS PAGE followed by western blot with 10442-1-AP(P53 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human endometrial cancer using 10442-1-AP(P53 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human endometrial cancer using 10442-1-AP(P53 Antibody) at Dilution 1:100 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | P53 fusion protein ag0698 | Full name | tumor protein p53 |
|
Calculated molecular weight |
44kd |
Observed molecular
weight |
53kd |
|
GenBank accession number |
BC003596 | Gene ID (NCBI) | 7157 |
| Gene symbol | TP53 | Synonyms | FLJ92943; LFS1; TRP53; p53 |
Background
TP53, also named as P53 and NY-CO-13, belongs to the p53 family. It has 9 isoforms. In SDS-Page, the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. |
