Tested Applications
| Positive WB detected in | HeLa cells, mouse heart tissue, human brain tissue, Jurkat cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
Product Information
14960-1-AP targets PABPC4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6813 Product name: Recombinant human PABPC4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 285-631 aa of BC065540 Sequence: QERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSITSAKVMLEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEERKAHLTNQYMQRVAGMRALPANAILNQFQPAAGGYFVPAVPQAQGRPPYYTPNQLAQMRPNPRWQQGGRPQGFQGMPSAIRQSGPRPTLRHLAPTGNAPASRGLPTTTQRVGVPTAVQNLAPRAAVAAAAPRAVAPYKYASSVRSPHPAIQPLQAPQPAVHVQGQEPLTASMLAAAPPQEQKQMLGERLFPLIQTMHSNLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHHAKKEAAQKVGAVAAATS Predict reactive species |
| Full Name | poly(A) binding protein, cytoplasmic 4 (inducible form) |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70-71 kDa |
| GenBank Accession Number | BC065540 |
| Gene Symbol | PABPC4 |
| Gene ID (NCBI) | 8761 |
| RRID | AB_2156897 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13310 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PABPC4 antibody 14960-1-AP | Download protocol |
| IHC protocol for PABPC4 antibody 14960-1-AP | Download protocol |
| IP protocol for PABPC4 antibody 14960-1-AP | Download protocol |
| WB protocol for PABPC4 antibody 14960-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. | ||
EMBO Rep LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma
| ||
J Immunol HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
| ||
Nat Struct Mol Biol Poised PABP-RNA hubs implement signal-dependent mRNA decay in development | ||
BMC Pregnancy Childbirth Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics. | ||
PLoS Pathog Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Abe (Verified Customer) (04-25-2024) | Worked well for IF at 1:500 overnight in 4c for RPE-1 cells.
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