Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 10639-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10639-1-AP
Mouse heart tissue lysates were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10639-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10639-1-AP (PACSIN3 antibody) at dilution of 1:100 (under 25x lens).
IHC staining of human heart using 10639-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 10639-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10639-1-AP
Immunofluorescent analysis of HeLa cells using 10639-1-AP (PACSIN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PACSIN3 Polyclonal antibody

PACSIN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10639-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein kinase C and casein kinase substrate in neurons protein 3, SH3 domain-containing protein 6511

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	mouse heart tissue, HeLa cells
Positive IHC detected in	human breast cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

WB	See 1 publications below

10639-1-AP targets PACSIN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1006 Product name: Recombinant human PACSIN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC007914 Sequence: MAPEEDAGGEALGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYAQQLADWARKWRGTVEKGPQYGTLEKAWHAFFTAAERLSALHLEVREKLQGQDSERVRAWQRGAFHRPVLGGFRESRAAEDGFRKAQKPWLKRLKEVEASKKSYHAARKDEKTAQTRESHAKADSAVSQEQLRKLQERVERCAKEAEKTKAQYEQTLAELHRYTPRYMEDMEQAFETCQAAERQRLLFFKDMLLTLHQHLDLSSSEKFHELHRDLHQGIEAASDEEDLRWWRSTHGPGMAMNWPQFE Predict reactive species
Full Name	protein kinase C and casein kinase substrate in neurons 3
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC007914
Gene Symbol	PACSIN3
Gene ID (NCBI)	29763
RRID	AB_2159453
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UKS6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for PACSIN3 antibody 10639-1-AP	Download protocol
IHC protocol for PACSIN3 antibody 10639-1-AP	Download protocol
WB protocol for PACSIN3 antibody 10639-1-AP	Download protocol