Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10085-1-AP targets PAFAH2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0124 Product name: Recombinant human PAFAH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 165-392 aa of BC001158 Sequence: WIPFRRVEEGEKEFHVRNPQVHQRVSECLRVLKILQEVTAGQTVFNILPGGLDLMTLKGNIDMSRVAVMGHSFGGATAILALAKETQFRCAVALDAWMFPLERDFYPKARGPVFFINTEKFQTMESVNLMKKICAQHEQSRIITVLGSVHRSQTDFAFVTGNLIGKFFSTETRGSLDPYEGQEVMVRAMLAFLQKHLDLKEDYNQWNNLIEGIGPSLTPGAPHHLSSL Predict reactive species |
| Full Name | platelet-activating factor acetylhydrolase 2, 40kDa |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa and 80 kDa |
| GenBank Accession Number | BC001158 |
| Gene Symbol | PAFAH2 |
| Gene ID (NCBI) | 5051 |
| RRID | AB_2160055 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99487 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Platelet-activating factor acetylhydrolase 2 (PAFAH2), also known as PAF-AH II, is an intracellular phospholipase A2 enzyme that hydrolyzes platelet-activating factor (PAF) and oxidatively fragmented phospholipids (PMID: 34116047). Belonging to the PAFAH family, PAFAH2 shares significant homology with plasma PAFAH (PLA2G7) but is distinct in its intracellular localization (PMID: 8955149). The enzyme features an α/β-hydrolase fold with a Ser-His-Asp catalytic triad and does not require Ca²⁺ for activity (PMID: 34116047). PAF-AH II expresses in almost all tissues, but predominantly in epithelial cells such as renal proximal and distal tubules, hepatocytes, and intestinal column epithelium. PAFAH2 isolated from mammalian cells was found to be both a monomer and dimer. In an unstressed situation, PAFAH2 as a dimer remains in the cytoplasm, and when exposed to oxidative stress, the homodimers of PAFAH2 dissociate into monomers to bind to membranes, ER, and Golgi apparatus (PMID: 34116047).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for PAFAH2 antibody 10085-1-AP | Download protocol |
| WB protocol for PAFAH2 antibody 10085-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
J Biol Chem Oxidatively truncated phospholipids are required agents of tumor necrosis factor α (TNFα)-induced apoptosis. | ||
J Lipid Res Intracellular PAF catabolism by PAF acetylhydrolase counteracts continual PAF synthesis. | ||
Biochemistry Trafficking of platelet-activating factor acetylhydrolase type II in response to oxidative stress. | ||
Biol Direct Platelet-activating factor induces ferroptosis by binding to ATF3 and inhibiting the SLC7A11/GPX4 axis to suppress the progression of endometrial carcinoma. | ||
ACS Chem Biol Phospholipase PAFAH2 Mediates Ferroptosis Surveillance and Lipid Remodeling to Promote Resistance in KEAP1 Mutant Cancers
|
