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KD/KO Validated

PAN2 Polyclonal antibody

PAN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16427-1-AP

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:3.1.13.4, hPAN2, Inactive ubiquitin carboxyl-terminal hydrolase 52, KIAA0710, PAB1P-dependent poly(A)-specific ribonuclease

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Proteintech Guarantee

Filter: AllWBIPIHCIF/ICC

WB analysis of NIH/3T3 using 16427-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16427-1-AP (PAN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 16427-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16427-1-AP (PAN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of NIH/3T3 using 16427-1-AP
IP result of anti-PAN2 (IP:16427-1-AP, 4ug; Detection:16427-1-AP 1:800) with NIH/3T3 cells lysate 1160 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16427-1-AP (PAN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16427-1-AP (PAN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 16427-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of NIH/3T3 using 16427-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PAN2 antibody (16427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	NIH/3T3 cells, HepG2 cells
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	mouse heart tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human liver tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16427-1-AP targets PAN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9670 Product name: Recombinant human PAN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 850-1198 aa of BC024043 Sequence: ATVVHILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFDMNWKVPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQIGDLVGLDAEFVTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLAWYFLDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPEGQTSPKNAAVFSSVLAL Predict reactive species
Full Name	PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
Calculated Molecular Weight	1198 aa, 135 kDa
Observed Molecular Weight	135 kDa
GenBank Accession Number	BC024043
Gene Symbol	PAN2
Gene ID (NCBI)	9924
RRID	AB_1851434
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q504Q3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for PAN2 antibody 16427-1-AP	Download protocol
IHC protocol for PAN2 antibody 16427-1-AP	Download protocol
IP protocol for PAN2 antibody 16427-1-AP	Download protocol
WB protocol for PAN2 antibody 16427-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

PABPC1 Antibody (Cat# 16427-1-AP) has 9 total citations published in journals including Nature Communications, Nature Structural & Molecular Biology, Cell Reports, Cells, Neurobiology of Disease, Translational Oncology, Biology of Reproduction, and PLoS ONE. Research fields span RNA deadenylation and mRNA decay, USP52-mediated deubiquitination, ferroptosis in head and neck carcinoma, tauopathy, oocyte maturation, and clear cell renal carcinoma progression. Applications include WB, IHC, IF, and IP in human and mouse models.

Species	Application	Title
human	WB,IHC,IF	Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. Authors - Shangda Yang KD Validated View Article
	WB	Nat Struct Mol Biol An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay. Authors - Stefanie Jonas View Article
mouse	WB	Cell Rep YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways. Authors - Jiadong Liu View Article
human	WB	Cell Rep USP52-RAB11FIP5 axis suppresses ferroptosis by repressing transferrin receptor recycling in head and neck squamous cell carcinoma Authors - Anjiang Sun View Article
mouse	WB	Cells Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN). Authors - Tian-Li Duan View Article
human	IF	Neurobiol Dis Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes. Authors - Rebecca L Kow View Article