Filter: AllWBIPIHCIF

WB analysis of HeLa using 24284-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 24284-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 24284-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 24284-1-AP
IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug.
IHC staining of human testis using 24284-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 24284-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 24284-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PAPOLG antibody (24284-1-AP) at dilution of 1:1600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

PAPOLG Polyclonal antibody

Name: PAPOLG Polyclonal antibody
Brand: Proteintech
SKU: 24284-1-AP

PAPOLG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24284-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PAP gamma, Neo-poly(A) polymerase, Neo-PAP, NeoPAP, Neo PAP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24284-1-AP targets PAPOLG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16208 Product name: Recombinant human PAPOLG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 469-736 aa of BC111701 Sequence: QANNINMLKEGMKIEATHVKKKQLHHYLPAEILQKKKKQSLSDVNRSSGGLQSKRLSLDSSCLDSSRDTDNGTPFNSPASKSDSPSVGETERNSAEPAAVIVEKPLSVPPAQGLSIPVIGAKVDSTVKTVSPPTVCTIPTVVGRNVIPRITTPHNPAQGQPHLNGMSNITKTVTPKRSHSPSIDGTPKRLKDVEKFIRLESTFKDPRTAEERKRKSVDAIGGESMPIPTIDTSRKKRLPSKELPDSSSPVPANNIRVIKNSIRLTLNR Predict reactive species
Full Name	poly(A) polymerase gamma
Calculated Molecular Weight	736 aa, 83 kDa
Observed Molecular Weight	83 kDa
GenBank Accession Number	BC111701
Gene Symbol	PAPOLG
Gene ID (NCBI)	64895
RRID	AB_2879471
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BWT3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.

Protocols

Product Specific Protocols
IF protocol for PAPOLG antibody 24284-1-AP	Download protocol
IHC protocol for PAPOLG antibody 24284-1-AP	Download protocol
IP protocol for PAPOLG antibody 24284-1-AP	Download protocol
WB protocol for PAPOLG antibody 24284-1-AP	Download protocol