PAPSS2 Polyclonal antibody

PAPSS2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15978-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

3'-phosphoadenosine-5'-phosphosulfate synthase, Adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenylylsulfate 3'-phosphotransferase, Adenylyl-sulfate kinase, ATPSK2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of HepG2 using 15978-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15978-1-AP (PAPSS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human liver using 15978-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15978-1-AP (PAPSS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 15978-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15978-1-AP (PAPSS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 15978-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15978-1-AP (PAPSS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human thyroid cancer using 15978-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15978-1-AP (PAPSS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 15978-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15978-1-AP (PAPSS2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (3)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, human liver tissue
Positive IHC detected in	mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15978-1-AP targets PAPSS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8766 Product name: Recombinant human PAPSS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC009894 Sequence: MSGIKKQKTENQQKSTNVVYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTISFALEEYLVSHAIPCYSLDGDNVRHGLNRNLGFSPGDREENIRRIAEVAKLFADAGLVCITSFISPFAKDRENARKIHESAGLPFFEIFVDAPLNICESRDVKGLYKRARAGEIKGFTGIDSDYEKPETPERVLKTNLSTVSDCVHQVVELLQEQNIVPYTIIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFDTLLDDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEF Predict reactive species
Full Name	3'-phosphoadenosine 5'-phosphosulfate synthase 2
Calculated Molecular Weight	614 aa, 70 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC009894
Gene Symbol	PAPSS2
Gene ID (NCBI)	9060
RRID	AB_2159498
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95340
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for PAPSS2 antibody 15978-1-AP	Download protocol
WB protocol for PAPSS2 antibody 15978-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human, mouse	WB	Cell Rep Med Neuroprotective response against the onset of ischemic stroke by upregulation of histone H3Y99 sulfation. Authors - Li Jiang View Article
		Discov Oncol Application of Mendelian randomization and bioinformatic analysis to construct a prognostic model for thyroid cancer and perform pan-cancer analysis Authors - Zhenrun Zhan View Article
		Hum Reprod PAPSS2-mediated BMPR1A sulfation in stromal cells regulating endometrial decidualization via autophagy. Authors - Duo Zi View Article