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PARP10 Polyclonal antibody

PARP10 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 26072-1-AP

Synonyms

PARP 10, PARP10

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PARP10 Polyclonal antibody (26072-1-AP)

    WB analysis of Jurkat using 26072-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using PARP10 Polyclonal antibody (26072-1-AP)

    WB analysis of HL-60 using 26072-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PARP10 Polyclonal antibody (26072-1-AP)

    WB analysis of MCF-7 using 26072-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PARP10 Polyclonal antibody (26072-1-AP)

    IP experiment of Jurkat using 26072-1-AP

    IP result of anti-PARP10 (IP:26072-1-AP, 4ug; Detection:26072-1-AP 1:500) with Jurkat cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PARP10 Polyclonal antibody (26072-1-AP)

    IF Staining of HepG2 using 26072-1-AP

    Immunofluorescent analysis of HepG2 cells using 26072-1-AP (PARP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HL-60 cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26072-1-AP is PARP10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARP10 fusion protein Ag23457
Full Name poly (ADP-ribose) polymerase family, member 10
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC136542
Gene Symbol PARP10
Gene ID (NCBI) 84875
RRIDAB_2880362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PARP10 antibody 26072-1-APDownload protocol
IF protocol for PARP10 antibody 26072-1-APDownload protocol
IP protocol for PARP10 antibody 26072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols