Tested Applications
| Positive WB detected in | HeLa cells, U2OS cells, SH-SY5Y cells |
| Positive IHC detected in | human kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55149-1-AP targets PARP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | poly (ADP-ribose) polymerase 2 |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | NM_005484 |
| Gene Symbol | PARP2 |
| Gene ID (NCBI) | 10038 |
| RRID | AB_10858796 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGN5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PARP2 antibody 55149-1-AP | Download protocol |
| IHC protocol for PARP2 antibody 55149-1-AP | Download protocol |
| WB protocol for PARP2 antibody 55149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Oncogene CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma. | ||
Cells Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development | ||
Eur J Med Chem Selective degradation of PARP2 by PROTACs via recruiting DCAF16 for triple-negative breast cancer. | ||
J Biol Chem Duplexed CeTEAM drug biosensors reveal determinants of PARP inhibitor selectivity in cells | ||
Nat Chem Biol Stabilization of AFF1 by PARylation ensures transcriptional restart after DNA damage
| ||
Eur J Med Chem Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Andres Joaquin (Verified Customer) (02-09-2026) | It works very well for detecting PARP2 in mouse tissues by western blot.
|
Hasan (Verified Customer) (12-07-2022) | WB analysis for U2OS cells (WT and PARP2KO) with PARP2 Ab (55149-1-AP) at dilution of 1:2000 overnight.
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