PAX8 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | IF, IHC, WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | SKOV-3 cells, HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, Raji cells |
| Positive IHC detected in | human ovary tumor |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
IP = Immunoprecipitation
IP result of anti-PAX8 (10336-1-AP for IP and Detection) with Hela cell.
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10336-1-AP(PAX8 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human ovary tumor using 10336-1-AP(PAX8 Antibody) at Dilution 1:50 (under 10x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | PAX8 fusion protein ag0306 | Full name | paired box 8 |
|
Calculated molecular weight |
48 kDa |
Observed molecular
weight |
48 kDa, 58 kDa |
|
GenBank accession number |
BC001060 | Gene ID (NCBI) | 7849 |
| Gene symbol | PAX8 | Synonyms |
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. |
