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WB analysis of mouse liver using 27103-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 27103-1-AP (PCDH24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PCDH24 Polyclonal antibody

Name: PCDH24 Polyclonal antibody
Brand: Proteintech
SKU: 27103-1-AP

PCDH24 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27103-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Protocadherin-24, PC-LKC, PCLKC, PC LKC, CDHR2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse liver tissue
Positive IHC detected in	human kidney tissue, human liver tissue, human small intestine tissue, mouse small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

27103-1-AP targets PCDH24 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25840 Product name: Recombinant human PCDH24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 381-680 aa of BC130282 Sequence: SPRIPIDDLTMVVYDPDKGSNGTFLLSLGGPDAEAFSVSPERAAGSASVQVLVRVSALVDYERQTAMAVQVVATDSVSQNFSVAMVTIHLRDINDHRPTFPQSLYVLTVPEHSATGSVVTDSIHATDPDTGAWGQITYSLLPGNGADLFQVDPVSGTVTVRNGELLDRESQAVYYLTLQATDGGNLSSSTTLQIHLLDINDNAPVVSGSYNIFVQEEEGNVSVTIQAHDNDEPGTNNSRLLFNLLPGPYSHNFSLDPDTGLLRNLGPLDREAIDPALEGRIVLTVLVSDCGEPVLGTKVN Predict reactive species
Full Name	protocadherin 24
Calculated Molecular Weight	1310 aa, 142 kDa
Observed Molecular Weight	140-150 kDa
GenBank Accession Number	BC130282
Gene Symbol	PCDH24
Gene ID (NCBI)	54825
RRID	AB_2880757
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BYE9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherin-24 (PCDH24, also known as CDHR2 and protocadherin LKC) was originally identified as a novel member of the human non-classical cadherin family. PCDH24 may play a role in cell-cell adhesion and contact inhibition in epithelial cells. PCDH24 expression is markedly reduced in cancers derived from liver and colon tissues, indicating that PCDH24 may play a role as a tumor suppressor protein (PMID: 23115611). The calculated molecular weight of PCDH24 is 142 kDa.

Protocols

Product Specific Protocols
IHC protocol for PCDH24 antibody 27103-1-AP	Download protocol
WB protocol for PCDH24 antibody 27103-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Transl Androl Urol Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples. Authors - Xuechao Li View Article