Tested Applications
| Positive WB detected in | HEK-293 cells, HEK-293T cells, PC-13 cells, mouse liver tissue |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4800 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24103-1-AP targets PCGF6 in WB, IHC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21124 Product name: Recombinant human PCGF6 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-352 aa of BC010235 Sequence: MEGVAVVTAGSVGAAKTEGAAALPPPPPPPVSPPALTPAPAAGEEGPAPLSETGAPGCSGSRPPELEPERSLGRFRGRFEDEDEELEEEEELEEEEEEEEEDMSHFSLRLEGGRQDSEDEEERLINLSELTPYILCSICKGYLIDATTITECLHTFCKSCIVRHFYYSNRCPKCNIVVHQTQPLYNIRLDRQLQDIVYKLVINLEEREKKQMHDFYKERGLEVPKPAVPQPVPSSKGRSKKVLESVFRIPPELDMSLLLEFIGANEGTGHFKPLEKKFVRVSGEATIGHVEKFLRRKMGLDPACQVDIICGDHLLEQYQTLREIRRAIGDAAMQDGLLVLHYGLVVSPLKIT Predict reactive species |
| Full Name | polycomb group ring finger 6 |
| Calculated Molecular Weight | 352 aa, 39 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC010235 |
| Gene Symbol | PCGF6 |
| Gene ID (NCBI) | 84108 |
| RRID | AB_2737369 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9BYE7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCGF6, also named as MBLR or RNF134, is a 350 amino acid protein, which contains one RING-type zinc finger. PCGF6 localizes in the nucleus and is widely expressed in many tissues. PCGF6 as a transcriptional repressor may modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| IHC protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| IP protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| WB protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell Stem Cell SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States. | ||
Sci Adv The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands. | ||
Nat Commun Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.
| ||
Nat Commun PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.
| ||
Nat Commun E2F6 initiates stable epigenetic silencing of germline genes during embryonic development. | ||
Elife Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness. |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aktan (Verified Customer) (12-24-2019) | The blot is subcellular fractionation of the cell nuclei using increasing concentrations of salt. Last two lanes are chromatin fractions. After electrophoresis and transfer, 5%BSA in PBST was used as blocker for 1h. The primary antibody is diluted in blocker 1:500 and the blot is incubated o/n at cold. The bands are detected using licor secondary antibodies and Licor imager.
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