ALIX; AIP1 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IF, IP|
|Species specificity||human, mouse, rat; other species not tested.|
|Cited species||human, mouse|
|Positive WB detected in||Jurkat cells|
|Positive IF detected in||Hela cells|
|Recommended dilution||WB: 1:1000-1:10000|
IP = Immunoprecipitation
- IP result of PDCD6IP antibody (12422-1-AP for IP and Detection) with Jurkat cell lysate.
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12422-1-AP(ALIX; AIP1 Antibody) at dilution of 1:2000
- Immunofluorescent analysis of Hela cells, using PDCD6IP antibody 12422-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||ALIX; AIP1 fusion protein ag3074||Full name||programmed cell death 6 interacting protein|
|BC020066||Gene ID (NCBI)||10015|
|Gene symbol||PDCD6IP||Synonyms||AIP1; Alix; DRIP4; HP95; MGC17003|
ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.