ALIX; AIP1 Antibody
Rabbit Polyclonal
Catalog number: 12422-1-AP
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Applications
| Tested applications | ELISA, WB, IF, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | Jurkat cells |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:1000-1:10000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of PDCD6IP antibody (12422-1-AP for IP and Detection) with Jurkat cell lysate.
Jurkat cells were subjected to SDS PAGE followed by western blot with 12422-1-AP(ALIX; AIP1 Antibody) at dilution of 1:2000
Immunofluorescent analysis of Hela cells, using PDCD6IP antibody 12422-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | ALIX; AIP1 fusion protein ag3074 | Full name | programmed cell death 6 interacting protein |
|
Calculated molecular weight |
868aa,96kd |
Observed molecular
weight |
96kd |
|
GenBank accession number |
BC020066 | Gene ID (NCBI) | 10015 |
| Gene symbol | PDCD6IP | Synonyms | AIP1; Alix; DRIP4; HP95; MGC17003 |
Background
ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. |
