PDCL Polyclonal antibody

PDCL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16057-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PDCL, PhLP, phosducin like, Phosducin like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIHC

WB analysis using 16057-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16057-1-AP (PDCL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of NCI-H1299 using 16057-1-AP
NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 16057-1-AP (PDCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 16057-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16057-1-AP (PDCL antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 16057-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16057-1-AP (PDCL antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 16057-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16057-1-AP (PDCL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, NCI-H1299 cells, Jurkat cells
Positive IHC detected in	human placenta tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16057-1-AP targets PDCL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9029 Product name: Recombinant human PDCL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC017227 Sequence: MTTLDDKLLGEKLQYYYSSSEDEDSDHEDKDRGRCAPASSSVPAEAELAGEGISVNTGPKGVINDWRRFKQLETEQREEQCREMERLIKKLSMTCRSHLDEEEEQQKQKDLQEKISGKMTLKEFAIMNEDQDDEEFLQQYRKQRMEEMRQQLHKGPQFKQVFEISSGEGFLDMIDKEQKSIVIMVHIYEDGIPGTEAMNGCMICLAAEYPAVKFCKVKSSVIGASSQFTRNALPALLIYKGGELIGNFVRVTDQLGDDFFAVDLEAFLQEFGLLPEKEVLVLTSVRNSATCHSEDSDLEID Predict reactive species
Full Name	phosducin-like
Calculated Molecular Weight	301 aa, 34 kDa
Observed Molecular Weight	40-42 kDa
GenBank Accession Number	BC017227
Gene Symbol	PDCL
Gene ID (NCBI)	5082
RRID	AB_2236943
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13371
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for PDCL antibody 16057-1-AP	Download protocol
WB protocol for PDCL antibody 16057-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
rat	WB	Acta Physiol (Oxf) ATP6V1A is required for synaptic rearrangements and plasticity in murine hippocampal neurons Authors - Alessandro Esposito View Article