Tested Applications
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse testis tissue, rat kidney tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18078-1-AP targets PDE10A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12771 Product name: Recombinant human PDE10A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 434-779 aa of BC104860 Sequence: KLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIGPFENMWPGIFVYMVHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDHPLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFGNRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAEGDEMKKLGIQPIPMMDRDKKDEVPQGQLGFYNAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSVAQKAAASED Predict reactive species |
| Full Name | phosphodiesterase 10A |
| Calculated Molecular Weight | 779 aa, 88 kDa |
| Observed Molecular Weight | 89 kDa |
| GenBank Accession Number | BC104860 |
| Gene Symbol | PDE10A |
| Gene ID (NCBI) | 10846 |
| RRID | AB_2878492 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y233 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDE10A (Phosphodiesterase 10A) is a single member of the PDE10 protein family, and it catalyzes both cAMP and cGMP hydrolysis (PMID: 34550322). The literature on PDE10A has been focused on psychiatric and neurological disorders because PDE10A expression is enriched in the striatal region of the brain under normal conditions (PMID: 12967715). Moreover, PDE10A expression is induced in various human tumor cell lines and PDE10A inhibition suppresses tumor cell growth (PMID: 37232184).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDE10A antibody 18078-1-AP | Download protocol |
| IHC protocol for PDE10A antibody 18078-1-AP | Download protocol |
| IP protocol for PDE10A antibody 18078-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDE10A Polyclonal antibody (Cat# 18078-1-AP) has 8 citations published in Neuron, Environmental International, EMBO Journal, Cell and Molecular Life Sciences, Biochemical Pharmacology, Journal of Neuroscience, and Andrology. Research fields span neurodegeneration (TAF15 aggregation), neuronal apoptosis, PET imaging of neuronal circuits, vascular development, bladder cancer and immune escape, striatal cAMP signaling and motor learning, and bovine reproductive biology (sperm phosphodiesterase activity).
| Species | Application | Title |
|---|---|---|
Environ Int Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms | ||
EMBO J A genetically targeted reporter for PET imaging of deep neuronal circuits in mammalian brains. | ||
Biochem Pharmacol N6-methyladenosine-modified RNF220 induces cisplatin resistance and immune escape via regulating PDE10A K48-linked ubiquitination in bladder cancer | ||
Cell Mol Life Sci Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation | ||
