Tested Applications
| Positive WB detected in | mouse brain tissue, mouse heart tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55306-1-AP targets PDE2A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | phosphodiesterase 2A, cGMP-stimulated |
| Calculated Molecular Weight | 106 kDa |
| Observed Molecular Weight | 100-106 kDa |
| GenBank Accession Number | NM_002599 |
| Gene Symbol | PDE2A |
| Gene ID (NCBI) | 5138 |
| RRID | AB_11182279 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00408 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDE2A, also named as cGSPDE and CGS-PDE, belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. It is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. This antibody is specific to PDE2A.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDE2A antibody 55306-1-AP | Download protocol |
| IP protocol for PDE2A antibody 55306-1-AP | Download protocol |
| WB protocol for PDE2A antibody 55306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDE2A antibody (Cat# 55306-1-AP) has 17 citations across journals including Sci Adv, Cell Rep, Br J Pharmacol, JCI Insight, Biochem Pharmacol, Cells, J Am Heart Assoc, Commun Biol, Front Pharmacol, Thromb Haemost, Int J Neuropsychopharmacol, Mol Nutr Food Res, ACS Chem Neurosci, FASEB J, Development, FASEB Bioadv, and Mol Metab. Research fields span Parkinson's disease, cardiac function, mitochondrial biology, hepatocellular carcinoma, mitophagy, depression, neuropathic pain, and hepatic lipid metabolism.
| Species | Application | Title |
|---|---|---|
Sci Adv RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease. | ||
Br J Pharmacol 17β-estradiol reduces mitochondrial cAMP content and cytochrome oxidase activity in a phosphodiesterase 2-dependent manner. | ||
JCI Insight Phosphodiesterase 2A as a therapeutic target to restore cardiac neurotransmission during sympathetic hyperactivity. | ||
Biochem Pharmacol Hydrogen sulfide-mediated stimulation of mitochondrial electron transport involves inhibition of the mitochondrial phosphodiesterase 2A, elevation of cAMP and activation of protein kinase A. | ||
J Am Heart Assoc Cardiac Gene Therapy With Phosphodiesterase 2A Limits Remodeling and Arrhythmias in Mouse Models of Heart Failure |
