PDLIM1,CLP36 Antibody
Rabbit Polyclonal
Catalog number: 11674-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IF, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, rat |
| Positive WB detected in | human lung tissue, human ileum tissue |
| Positive IHC detected in | human pancreas cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
human lung tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP(PDLIM1,CLP36 Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human pancreas cancer using 11674-1-AP(PDLIM1,CLP36 Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using 11674-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | PDLIM1,CLP36 fusion protein ag2274 | Full name | PDZ and LIM domain 1 |
|
Calculated molecular weight |
329aa,36kd |
Observed molecular
weight |
36kd |
|
GenBank accession number |
BC000915 | Gene ID (NCBI) | 9124 |
| Gene symbol | PDLIM1 | Synonyms | CLIM1; CLP-36; CLP36; hCLIM1 |
Background
PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the -actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583) |
