Tested Applications
| Positive WB detected in | HT-1080 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18796-1-AP targets PDS5B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8301 Product name: Recombinant human PDS5B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 228-529 aa of BC039256 Sequence: AIEPYITNFFNQVLMLGKTSISDLSEHVFDLILELYNIDSHLLLSVLPQLEFKLKSNDNEERLQVVKLLAKMFGAKDSELASQNKPLWQCYLGRFNDINVPIRLECVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVSIVTAAKKDILLVNDHLLNFVRERTLDKRWRVRKEAMMGLAQIYKKYALQSAAGKDAAKQIAWIKDKLLHIYYQNSIDDRLLVERIFAQYMVPHNLETTERMKCLYYLYATLDLNAVKYVSNIKFCSFHPLQYIGFYGKETTNTCILKCNLCSVNIV Predict reactive species |
| Full Name | PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) |
| Calculated Molecular Weight | 1391 aa, 158 kDa, 165 kDa |
| Observed Molecular Weight | 165-170 kDa |
| GenBank Accession Number | BC039256 |
| Gene Symbol | PDS5B |
| Gene ID (NCBI) | 23047 |
| RRID | AB_10642562 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NTI5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDS5B antibody 18796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Zhenmin (Verified Customer) (08-19-2020) | We tested IF staining with this primary antibody on mouse testis. It showed very specific nuclear staining in testicular germ cells. This application was not indicated in the data sheet.
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