Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
13435-1-AP targets Twinkle/PEO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4239 Product name: Recombinant human PEO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 154-506 aa of BC033762 Sequence: LVRPGDQQPRPLEALNGGFNLSRILRTALPAWHKSIVSFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLCSQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK Predict reactive species |
| Full Name | chromosome 10 open reading frame 2 |
| Calculated Molecular Weight | 684 aa, 77 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC033762 |
| Gene Symbol | PEO1 |
| Gene ID (NCBI) | 56652 |
| RRID | AB_10644286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RR1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Twinkle, also named as C10orf2, PEO1, is a 684 amino acid protein, which forms multimers in vitro, including hexamers. Twinkle colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression. Twinkle involves in mitochondrial DNA (mtDNA) metabolism and could function as an adenine nucleotide-dependent DNA helicase. Twinkle exists various isoform and molecular weight of isoform is 60 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Twinkle/PEO1 antibody 13435-1-AP | Download protocol |
| WB protocol for Twinkle/PEO1 antibody 13435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis. | ||
EMBO J ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA. | ||
Cell Rep Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology. | ||
Cell Death Differ Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization. | ||
Nucleic Acids Res A deafness-associated mitochondrial DNA mutation caused pleiotropic effects on DNA replication and tRNA metabolism |
