Tested Applications
| Positive WB detected in | Daudi cells, MCF-7 cells, K-562 cells, HeLa cells |
| Positive IHC detected in | human pancreas tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18793-1-AP targets Twinkle/PEO1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6628 Product name: Recombinant human PEO1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 154-506 aa of BC033762 Sequence: LVRPGDQQPRPLEALNGGFNLSRILRTALPAWHKSIVSFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLCSQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK Predict reactive species |
| Full Name | chromosome 10 open reading frame 2 |
| Calculated Molecular Weight | 684 aa, 77 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC033762 |
| Gene Symbol | PEO1 |
| Gene ID (NCBI) | 56652 |
| RRID | AB_2066354 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RR1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Twinkle, also named as Progressive external ophthalmoplegia 1 protein is a 684 amino acid protein, which involves in mitochondrial DNA (mtDNA) metabolism. The predicted full-length protein is 684 amino acids, with amolecular mass of 77 kD, whereas the splice variant mRNAencodes a 66-kD product of 582 amino acids, lacking residues 579-684 and terminating with four unique amino acids (PMID: 11431692).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Twinkle/PEO1 antibody 18793-1-AP | Download protocol |
| WB protocol for Twinkle/PEO1 antibody 18793-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Twinkle/PEO1 polyclonal antibody (Cat# 18793-1-AP) has 2 citations. These appear in Signal Transduction and Targeted Therapy and Scientific Reports. The research fields encompass mitochondrial DNA (mtDNA) demethylation and leakage fueling antitumor immunity in colorectal cancer, as well as mtDNA nucleoid maintenance. Both studies utilized the antibody for Western blot applications, with one in mouse and one in human samples.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer. | ||
