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PGM1 Polyclonal antibody

PGM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15161-1-AP

Publications(20)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Phosphoglucomutase-1, GSD14, EC:5.4.2.2, CDG1T

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIPIHCIF/ICC

WB analysis using 15161-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15161-1-AP (PGM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 15161-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15161-1-AP (PGM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 15161-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15161-1-AP (PGM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse skin using 15161-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 15161-1-AP (PGM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse skin using 15161-1-AP
IP result of anti-PGM1 (IP:15161-1-AP, 4ug; Detection:15161-1-AP 1:1000) with mouse skin tissue lysate 3200ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 15161-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15161-1-AP (PGM1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 15161-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15161-1-AP (PGM1 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 15161-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15161-1-AP (PGM1 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 15161-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15161-1-AP (PGM1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 15161-1-AP
Immunofluorescent analysis of HepG2 cells, using PGM1 antibody 15161-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 15161-1-AP
Immunofluorescent analysis of HepG2 cells, using PGM1 antibody 15161-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (20)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, mouse skin tissue, HEK-293 cells, Jurkat cells, mouse heart tissue, rat heart tissue
Positive IP detected in	mouse skin tissue
Positive IHC detected in	human hepatocirrhosis tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15161-1-AP targets PGM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7269 Product name: Recombinant human PGM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 213-562 aa of BC001756 Sequence: SGPNRLKICIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKHGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT Predict reactive species
Full Name	phosphoglucomutase 1
Calculated Molecular Weight	61 kDa
Observed Molecular Weight	61 kDa
GenBank Accession Number	BC001756
Gene Symbol	PGM1
Gene ID (NCBI)	5236
RRID	AB_2161415
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P36871
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for PGM1 antibody 15161-1-AP	Download protocol
IHC protocol for PGM1 antibody 15161-1-AP	Download protocol
IP protocol for PGM1 antibody 15161-1-AP	Download protocol
WB protocol for PGM1 antibody 15161-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB,IF	Cell Metab Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis. Authors - Marion Curtis KD Validated View Article
human	WB	Brain C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis. Authors - Scott P Allen View Article
human	WB	J Hazard Mater Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes. Authors - Ye Zhu View Article
human	WB	Cancer Lett Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis. Authors - Xintian Chen View Article
human	IP	Biochim Biophys Acta Mol Basis Dis Sec13 promotes glycolysis by inhibiting Ubqln1 mediated Pgm1 ubiquitination in ALI Authors - Dongdong Wu View Article
human	WB	Front Cell Dev Biol Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma Authors - Wenshuo Zhang View Article