PHGDH Antibody
Rabbit Polyclonal
Catalog number: 14719-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human lymphoma |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 14719-1-AP(PHGDH Antibody) at dilution of 1:1200
Immunohistochemistry of paraffin-embedded human lymphoma using 14719-1-AP(PHGDH Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lymphoma using 14719-1-AP(PHGDH Antibody) at Dilution 1:100 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | PHGDH fusion protein ag6445 | Full name | phosphoglycerate dehydrogenase |
|
Calculated molecular weight |
57kd |
Observed molecular
weight |
57kd |
|
GenBank accession number |
BC000303 | Gene ID (NCBI) | 26227 |
| Gene symbol | PHGDH | Synonyms | 3-PGDH; 3PGDH; MGC3017; PDG; PGAD; PGD; PGDH; SERA |
Background
PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ). |
