Tested Applications
| Positive WB detected in | A375 cells, HL-60 cells, K-562 cells, mouse ovary tissue, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14242-1-AP targets PIAS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5498 Product name: Recombinant human PIAS4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 210-510 aa of BC029874 Sequence: PNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC Predict reactive species |
| Full Name | protein inhibitor of activated STAT, 4 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57-70 kDa |
| GenBank Accession Number | BC029874 |
| Gene Symbol | PIAS4 |
| Gene ID (NCBI) | 51588 |
| RRID | AB_2164365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N2W9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members (PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PIAS4 antibody 14242-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PIAS4 Polyclonal antibody (Cat# 14242-1-AP) has 15 citations across 12 journals including Nature Communications, Signal Transduction and Targeted Therapy, PLoS Biology, FASEB Journal, and Oncology Reports. Research fields span SUMOylation biology, cancer (leukemia, metastasis), respiratory diseases (asthma, airway inflammation), cardiovascular disease (aortic aneurysm), metabolic disorders (diabetic cardiomyopathy), viral infection (SARS-CoV-2), DNA damage response, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting ZMIZ1 induces differentiation in acute myeloid leukemia via chromatin remodeling. | ||
Nat Commun Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA.
| ||
Nat Commun Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy
| ||
Nat Commun Reprogramming gut microenvironment for the treatment of acute severe ulcerative colitis via a synergistic therapy of necroptosis blockade and organoid transplantation. | ||
PLoS Biol DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program.
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