PIK3C2B Polyclonal antibody

PIK3C2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24788-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C2 PI3K, C2-PI3K, EC:2.7.1.137, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta, Phosphoinositide 3-kinase-C2-beta

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICC

WB analysis using 24788-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24788-1-AP (PIK3C2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 24788-1-AP
IP result of anti-PIK3C2B (IP:24788-1-AP, 4ug; Detection:24788-1-AP 1:1500) with HeLa cells lysate 1240 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 24788-1-AP
IP result of anti-PIK3C2B (IP:24788-1-AP, 5ug; Detection:24788-1-AP 1:800) with HeLa cells lysate 2500ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skeletal muscle using 24788-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24788-1-AP (PIK3C2B Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skeletal muscle using 24788-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24788-1-AP (PIK3C2B Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 24788-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24788-1-AP (PIK3C2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24788-1-AP targets PIK3C2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20474 Product name: Recombinant human PIK3C2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC132876 Sequence: MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHDKEENRAKQNADPSLISWDEPGVDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSLSPQEGPPNHSTSQGPQPGSDPWPKGSLSGDYLYIFDGSDGGVSSSPGPGDIEGSCKKLSPPPLPPRASIWDTPPLPPRKGSPSSSKISQPSDINTFSLVEQLPGKLLEHRILEEEEVLGGGGQGRLLGSVDYDGINDAITRLNLKSTYDAEMLRDATRGWKEGRGPLDFSKDTSGKPVARSKTMPPQVPPRTYASRYGNRKNATPGKNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRSGS Predict reactive species
Full Name	phosphoinositide-3-kinase, class 2, beta polypeptide
Calculated Molecular Weight	1634 aa, 185 kDa
Observed Molecular Weight	160-185 kDa
GenBank Accession Number	BC132876
Gene Symbol	PIK3C2B
Gene ID (NCBI)	5287
RRID	AB_2879725
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00750
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for PIK3C2B antibody 24788-1-AP	Download protocol
IHC protocol for PIK3C2B antibody 24788-1-AP	Download protocol
IP protocol for PIK3C2B antibody 24788-1-AP	Download protocol
WB protocol for PIK3C2B antibody 24788-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human		Anal Chem Pseudotargeted MS Method for the Sensitive Analysis of Protein Phosphorylation in Protein Complexes. Authors - Jiawen Lyu View Article
mouse	WB	JCI Insight Pharmacological PIK3C2B inhibition rescues XLMTM phenotype in mouse models and identifies molecular markers of disease. Authors - Andrew Shearer KD Validated View Article
human	WB	Cell Rep ALKBH5 causes retinal pigment epithelium anomalies and choroidal neovascularization in age-related macular degeneration via the AKT/mTOR pathway Authors - Ru-Xu Sun View Article
human	WB,IHC	Biochem Biophys Rep PIK3C2B drives lung cancer progression through coordinating metabolic reprogramming and EMT-mediated metastasis. Authors - Xinyue Chou KD Validated View Article