Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells |
| Positive IHC detected in | human pancreas cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13218-1-AP targets PIP4K2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4099 Product name: Recombinant human PIP4K2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-281 aa of BC027459 Sequence: MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEEILVLSPGRRIA Predict reactive species |
| Full Name | phosphatidylinositol-5-phosphate 4-kinase, type II, beta |
| Calculated Molecular Weight | 416 aa, 47 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC027459 |
| Gene Symbol | PIP4K2B |
| Gene ID (NCBI) | 8396 |
| RRID | AB_10644482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78356 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer. It binds TNFRSF1A(24/32/50kd).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PIP4K2B antibody 13218-1-AP | Download protocol |
| WB protocol for PIP4K2B antibody 13218-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Nat Commun MANF regulates hypothalamic control of food intake and body weight.
| ||
Eur J Med Chem Design, synthesis and biological evaluation of the activity-based probes for FGFR covalent inhibitor | ||
JBMR Plus Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament |
