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KD/KO Validated

PIP4K2C Polyclonal antibody

Name: PIP4K2C Polyclonal antibody
Brand: Proteintech
SKU: 17077-1-AP

PIP4K2C Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17077-1-AP

Synonyms

PI(5)P 4 kinase type II gamma, PIP4K2C, PIP4KII gamma, PIP5K2C

Validation Data Gallery

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AllWBIHC

WB analysis using 17077-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 17077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 17077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 17077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17077-1-PBS
HeLa cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17077-1-PBS
HepG2 cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17077-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 17077-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 17077-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 17077-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, PC-12 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 3 publications below

Product Information

17077-1-AP targets PIP4K2C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PIP4K2C fusion protein Ag10989
Full Name	phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
Calculated Molecular Weight	421 aa, 47 kDa
Observed Molecular Weight	47 kDa
GenBank Accession Number	BC028596
Gene Symbol	PIP4K2C
Gene ID (NCBI)	79837
RRID	AB_2715526
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIP4K2C is involved in several processes, including 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process; negative regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity; and positive regulation of autophagosome assembly. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism.

Protocols

Product Specific Protocols
WB protocol for PIP4K2C antibody 17077-1-AP	Download protocol
IHC protocol for PIP4K2C antibody 17077-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Elife Inhibition of PIP4Kγ ameliorates the pathological effects of mutant huntingtin protein. Authors - Ismael Al-Ramahi KD Validated View Article
human	WB	Cell Rep PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism. Authors - Diana G Wang KO Validated View Article
human	WB	Autophagy Rep Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration Authors - Ron Benyair View Article

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PIP4K2C Polyclonal antibody

PIP4K2C Polyclonal Antibody for IHC, WB, ELISA

Validation Data Gallery

WB analysis using 17077-1-AP

WB analysis using 17077-1-AP

WB analysis using 17077-1-AP

WB analysis using 17077-1-AP

WB analysis of HeLa using 17077-1-PBS

WB analysis of HepG2 using 17077-1-PBS

WB analysis of HepG2 using 17077-1-AP

IHC staining of human liver cancer using 17077-1-AP

IHC staining of human liver cancer using 17077-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Inhibition of PIP4Kγ ameliorates the pathological effects of mutant huntingtin protein.

PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism.

Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration