PLA2G12A Polyclonal antibody

PLA2G12A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16009-1-AP

Synonyms

GXII, GXII sPLA2, phospholipase A2, group XIIA, PLA2G12, PLA2G12A, ROSSY, sPLA2 XII, UNQ2519/PRO6012



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, HEK-293 cells, mouse heart tissue, mouse kidney tissue
Positive IHC detected inhuman colon cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16009-1-AP targets PLA2G12A in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLA2G12A fusion protein Ag8722
Full Name phospholipase A2, group XIIA
Calculated Molecular Weight 189 aa, 21 kDa
Observed Molecular Weight 21-24 kDa
GenBank Accession NumberBC017218
Gene Symbol PLA2G12A
Gene ID (NCBI) 81579
RRIDAB_2164314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G12A (or PLA2G12) gene encodes group XII secreted phospholipase A2 (sPLA2) which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Cellular arachidonate (AA) release and prostaglandin (PG) production is regulated by sPLA(2)s, groups III and XII. Group XII sPLA2 have relatively low specific activity and are structurally and functionally distinct from other sPLA2s. Cells transfected with group XII sPLA(2) exhibited abnormal morphology, suggesting a unique functional aspect of this enzyme. Role of sPLA2s as potential tumour biomarkers and therapeutic targets for various cancers have been reported.

Protocols

Product Specific Protocols
WB protocol for PLA2G12A antibody 16009-1-APDownload protocol
IHC protocol for PLA2G12A antibody 16009-1-APDownload protocol
IF protocol for PLA2G12A antibody 16009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Cardiol

Phospholipase A2 expression in coronary thrombus is increased in patients with recurrent cardiac events after acute myocardial infarction.

Authors - Takahashi Soichiro S
mouseIHC,WB

Atherosclerosis

Quantitative trait locus mapping in mice identifies phospholipase Pla2g12a as novel atherosclerosis modifier.

Authors - Alexandros Nicolaou
ratWB

Exp Ther Med

Therapeutic effect of irbesartan combined with atorvastatin calcium in the treatment of rats with coronary heart disease.

Authors - Tao Li
humanIHC

J Atheroscler Thromb

The Expression of Groups IIE and V Phospholipase A2 is Associated with an Increased Expression of Osteogenic Molecules in Human Calcified Aortic Valves.

Authors - Koji Suzuki