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PLCD1 Polyclonal antibody

PLCD1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14228-1-AP

Synonyms

Phospholipase C delta 1, Phospholipase C III, phospholipase C, delta 1, PLC delta 1, PLC III, PLCD1

Validation Data Gallery

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  • Western Blot (WB) analysis of human rectum tissue using PLCD1 Polyclonal antibody (14228-1-AP)

    WB analysis of human rectum using 14228-1-AP

    human rectum tissue were subjected to SDS PAGE followed by western blot with 14228-1-AP (PLCD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PLCD1 Polyclonal antibody (14228-1-AP)

    WB analysis of human brain using 14228-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14228-1-AP (PLCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLCD1 Polyclonal antibody (14228-1-AP)

    IHC staining of human breast cancer using 14228-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14228-1-AP (PLCD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLCD1 Polyclonal antibody (14228-1-AP)

    IHC staining of human breast cancer using 14228-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14228-1-AP (PLCD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman rectum tissue, human brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14228-1-AP targets PLCD1 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCD1 fusion protein Ag5468
Full Name phospholipase C, delta 1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC050382
Gene Symbol PLCD1
Gene ID (NCBI) 5333
RRIDAB_2163278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PLCD1 antibody 14228-1-APDownload protocol
IHC protocol for PLCD1 antibody 14228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols