PLEKHG5 Polyclonal antibody

PLEKHG5 Polyclonal Antibody for WB, ELISA

Cat No. 19830-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DSMA4, GEF720, PH domain-containing family G member 5, Pleckstrin homology domain-containing family G member 5, RP4 650H14.3

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis of mouse heart using 19830-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 19830-1-AP (PLEKHG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A549 using 19830-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 19830-1-AP (PLEKHG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

mouse heart tissue, A549 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

19830-1-AP targets PLEKHG5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11725 Product name: Recombinant human PLEKHG5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 597-930 aa of BC015231 Sequence: KDSKMDVYCFLFTDLLLVTKAVKKAERTRVIRPPLLVDKIVCRELRDPGSFLLIYLNEFHSAVGAYTFQASGQALCRGWVDTIYNAQNQLQQLRAQEPPGSQQPLQSLEEEEDEQEEEEEEEEEEEEGEDSGTSAASSPTIMRKSSGSPDSQHCASDGSTETLAMVVVEPGDTLSSPEFDSGPFSSQSDETSLSTTASSATPTSELLPLGPVDGRSCSMDSAYGTLSPTSLQDFVAPGPMAELVPRAPESPRVPSPPPSPRLRRRTPVQLLSCPPHLLKSKSEASLLQLLAGAGTHGTPSAPSRSLSELCLAVPAPAQEADPGPALPNQDHPAA Predict reactive species
Full Name	pleckstrin homology domain containing, family G (with RhoGef domain) member 5
Calculated Molecular Weight	117 kDa
Observed Molecular Weight	105-125 kDa
GenBank Accession Number	BC015231
Gene Symbol	PLEKHG5
Gene ID (NCBI)	57449
RRID	AB_10858324
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94827
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for PLEKHG5 antibody 19830-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
		Sci Signal RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms. Authors - Junning Yang View Article
mouse	WB	J Neurosci NG2 Regulates Directional Migration of Oligodendrocyte Precursor Cells via Rho GTPases and Polarity Complex Proteins. Authors - Binamé Fabien F View Article
human	WB	FASEB J RND3 Inhibits Endometriosis Progression by Regulating Autophagy and Oxidative Stress Through PLEKHG5. Authors - Han Li KD Validated View Article
mouse	WB	FASEB J Characterization and computational simulation of human Syx, a RhoGEF implicated in glioblastoma Authors - Ryan J Boyd View Article